human esophageal epithelial cells heecs  (Procell Inc)

Journal: Journal of Cancer

Article Title: Identification and Characterization of Oxidative Stress and Endoplasmic Reticulum Stress-Related Genes in Esophageal Cancer

doi: 10.7150/jca.104376

Figure Lengend Snippet: TFRC promotes the proliferation, migration, and invasion of ESCA cells. A TFRC expression levels were analyzed in ESCA cells and HEEC using qRT-PCR. B The transfection efficiency of TFRC overexpression in TE1 cells and knockdown in OE33 cells was confirmed by qRT-PCR. C-D Cell proliferation was evaluated in TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells using MTS assays (C) and colony formation assays (D). E-F The migration and invasion capabilities of TFRC-overexpressing TE1 cells and TFRC-knockdown OE33 cells were assessed through transwell migration (E) and invasion (F) assays. Data are presented as the mean ± SD from at least three independent experiments. *P < 0.05.

Article Snippet: Human ESCC cell lines (KYSE150 and TE1), an EAC cell line (OE33), and a human normal esophageal epithelial cell line (HEEC) were obtained from the China Center for Type Culture Collection (CCTCC, Wuhan, China).

Techniques: Migration, Expressing, Quantitative RT-PCR, Transfection, Over Expression, Knockdown

Journal: Thoracic Cancer

Article Title: circ‐ TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy‐Mediated Radiosensitivity Through miR ‐145‐5p/ SIRT1 Axis

doi: 10.1111/1759-7714.15494

Figure Lengend Snippet: The expression of circ‐TTC17 and miR‐145‐5p in ESCC. (A–B) QRT‐PCR results indicated that circ‐TTC17 was highly expressed in ESCC tissues (T) and cell lines compared with that in adjacent normal tissues (N) and HEEC cells, respectively. * p < 0.05.

Article Snippet: ESCC cell lines (TE1, KYSE150, KYSE180, and KYSE30) and human esophageal epithelial cells (HEEC) were purchased from Procell (Wuhan, China) and grown in RPMI‐1640 medium containing 10% FBS and 1% penicillin/streptomycin. circ‐TTC17 siRNA, lentiviral shRNA, miR‐145‐5p mimic, anti‐miR‐145‐5p, and negative controls were transfected into KYSE180 and KYSE30 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: Thoracic Cancer

Article Title: circ‐ TTC17 Promotes Esophagus Squamous Cell Carcinoma Cell Growth, Metastasis, and Inhibits Autophagy‐Mediated Radiosensitivity Through miR ‐145‐5p/ SIRT1 Axis

doi: 10.1111/1759-7714.15494

Figure Lengend Snippet: miR‐145‐5p targeted SIRT1 in ESCC. (A) The binding sequences between miR‐145‐5p and SIRT1 3′UTR were presented. The interaction was verified using dual‐luciferase reporter assay (B–C) and RIP assay (D–E). (F) QRT‐PCR was used to detect SIRT1 mRNA expression in ESCC tissues (T) and adjacent normal tissues (N). (G) The correlation between SIRT1 and miR‐145‐5p expression was assessed by Pearson correlation coefficient analysis. (H) The protein expression of SIRT1 in T and N was determined by WB analysis. The mRNA and protein levels of SIRT1 in ESCC cell lines and HEEC cells were measured using qRT‐PCR (I) and WB analysis (J), respectively. (K) The transfection efficiency of miR‐145‐5p mimic and inhibitor was evaluated using qRT‐PCR. (L) SIRT1 protein expression in KYSE180 and KYSE30 cells was determined by WB analysis. * p < 0.05.

Article Snippet: ESCC cell lines (TE1, KYSE150, KYSE180, and KYSE30) and human esophageal epithelial cells (HEEC) were purchased from Procell (Wuhan, China) and grown in RPMI‐1640 medium containing 10% FBS and 1% penicillin/streptomycin. circ‐TTC17 siRNA, lentiviral shRNA, miR‐145‐5p mimic, anti‐miR‐145‐5p, and negative controls were transfected into KYSE180 and KYSE30 cells by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Transfection